Carcinogenesis, Teratogenesis & Mutagenesis ›› 2006, Vol. 18 ›› Issue (1): 16-018.doi: 10.3969/j.issn.1004-616x.2006.01.005

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Inhibition Effect of Betulin on Oesophageal Cancer EC109 Cell Line

CAI Wei-jia1, MA Yun-qing2, QI Yi-mei3, NIU Yong-dong, XU Li-yan1, LI Jian-xin1,3,, LI En-min1   

  1. 1. Medical College, Shantou University, Shantou 515031,China;2. Jilin City Experiment High School, Jilin 132011,China;3. Chemistry faculty, Northeast Normal University, Changchun 130024, China
  • Received:2004-12-29 Revised:2005-08-30 Online:2006-01-30 Published:2006-01-30
  • Contact: LI Jian-xin or LI En-min

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Abstract: BACKGROUND ＆ AIM: Betulin is an important triterpene. We investigated the antitumor activity of betulin, extracted from the bark of silver birch (Betula platyphylla Suk). MATERIAL AND METHODS: Betulin was isolated from dry powder of the bark of silver birch (Betula platyphylla Suk) by using ethanol circumfluence, decompress concentration and methanol-chloroform recrystallization. The inhibition rate on EC109 cell growth was detected by MTT (Methylthiazoletetrazolium) method, after the oesophageal cancer EC109 cells have been treated for 48h with different doses of betulin. RESULTS: With the increasing doses of betulin, the inhibition rate of EC109 cell growth was increased, and their morphological characteristics were changed significamtly. The inhibition rate showed dose-dependent relation. CONCLUSION: Betulin had potent inhibiting effects on EC109 cells growth in vitro.

Key words: betulin, oesophageal cancer cells, antitumor activity

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